Adjust the Gray Value Range to narrow or widen the interval, within which the gray values brighten from black to white.

Move the slider or directly enter specific numbers to change the interval. In the following images, observe the difference with varying settings:

By setting the gray value range from 0 to 255, the gray values are shown for the full data range.

When the gray value range is selected from 100 to 255, the gray values between 0 and 100 are assigned the color black, while the rest becomes relatively darker.

The higher range of gray values is taken out of the image when setting the gray values range from 0 to 100. The voxels with gray values from 0 to 100 become relatively brighter, whereas those with values 101 and higher are assigned the color white.

From the Overlay pull-down menu select the desired overlay for the 2D Slice Visualization section. The selection is also fitted automatically, if the corresponding options in the Image Processing and Segmentation section are edited.

The available overlays are None, Crop, Rotate, Shear, Ring Removal, Slice Alignment, Segmentation, AI-Labels, AI-Preview, and AI-Mixed.

If None is selected, no overlay is displayed in the 2D Slice Visualization section.

The first five options after None correspond to functions from the Image Processing tab:

• The option Crop corresponds to the feature Image Size – Crop. If selected, a yellow rectangle displays the cutout resulting from the entered values.
• The option Rotate corresponds to the feature Image Alignment – Rotation and displays the new axes after rotation, if the visualization of the axes is turned on, as described here.
• The overlay Shear corresponds to the option Image Alignment – Shear and shows the new resulting center line as a yellow line.
• If Ring Removal is selected, a yellow ring is displayed on the images, visualizing the values entered for the option CT Filters – Ring-Artifact Removal.
• The overlay Slice Alignment corresponds to the feature FIB-SEM Filters – Slice Alignment (Z-Direction). If selected, a red rectangle in the X-Y-plane displays the cutout resulting from the entered values.

The last four overlay options correspond to functions from the Segmentation & Labeling tab:

• The overlay Segmentation is selected by default and shows the segmentation result if the Visibility is turned on. The visualized segmentation corresponds to the values entered for the options in the Image Segmentation section.
• The overlays AI-Labels, AI-Preview, and AI-Mixed correspond features from the AI-based Segmentation method and are shown if the Visibility is turned on.

The slider for Visibility and the checkbox for Outline control the visualization of the threshold(s) used for segmentation as well as the labels and previews for the AI-based Segmentation. By moving the Visibility slider, the transparency of the threshold(s) is changed in the 2D Slice Visualization section.

When checking Outline, only the interface between segmented phases is overlaid with the given thresholding color(s). In the following images, observe the difference with varying settings:

When the Visibility is zero, the thresholded image is not visible and only the image gray values are shown. With intermediate Visibility, an overlay of the thresholded image and the gray value image is shown. With Visibility at its maximum, only the thresholded image is shown.

With Outline checked and intermediate Visibility, the interface between segmented phases is slightly overlaid with the given thresholding color. With Outline checked and increasing Visibility, these interfaces become increasingly highlighted.

By pressing CTRL + A you can toggle between the currently selected Visibility value and a Visibility value of zero. This allows you to set a defined overlay visibility and disable this overlay quickly with one shortcut, which can be especially helpful during labeling for AI segmentation.